Wanning

1、Educational Experience

(1) 2017/09-2022/06, China Pharmaceutical University, Key Laboratory of Drug Metabolism and Pharmacokinetics, Ph D

(2) 2013/09-2017/06, China Pharmaceutical University, School of Pharmacy, Bachelor

2、Working Experience

2022/06 to now, China Pharmaceutical University, School of Pharmacy, Post-doctor

1. Wan N‡, Wang N‡, Yu S, Zhang H, Tang S, Wang D, Lu W, Li H, Delafield DG, Kong Y, Wang X, Shao C, Lv L, Wang G, Tan R, Wang N*, Hao H*, Ye H*. Cyclic immonium ion of lactyllysine reveals widespread lactylation in the human proteome. Nat Methods. 2022, 19(7):854-864. (IF: 47.990) 

2. Yan W, Wang D, Wan N, Wang S, Shao C, Zhang H, Zhao Z, Lu W, Tian Y, Ye H*, Hao H*. Living Cell-Target Responsive Accessibility Profiling Reveals Silibinin Targeting ACSL4 for Combating Ferroptosis. Anal Chem. 2022, 94(43):14820- 14826. (IF 8.008)

3. Zhu Y‡, Wan N‡, Shan X, Deng G, Xu Q, Ye H*, Sun Y*. Celastrol targets adenylyl cyclase-associated protein 1 to reduce macrophages-mediated inflammation and ameliorates high fat diet-induced metabolic syndrome in mice. Acta Pharm Sin B (Cover Article). 2021, 11(5):1200-1212. (IF: 14.903)

(1) Chemoproteomics-based target discovery

(2) The proteomic landscape of lysine lactylation

1、Research Projects

(1) National Key Research and Development Program, Metabolic Regulation- Oriented Microbiome-Host-Drug Interaction Network and Signal Transduction Mechanism, 2021-2026, Participate.

2、Representative Research Achievements

Lactylation was initially discovered on human histones. Given its nascence, its occurrence on nonhistone proteins and downstream functional consequences remain elusive. Here we report a cyclic immonium ion of lactyllysine formed during tandem

mass spectrometry that enables confident protein lactylation assignment. We validated the sensitivity and specificity of this ion for lactylation through affinity-enriched lactylproteome analysis and large-scale informatic assessment of nonlactylated spectral libraries. With this diagnostic ion-based strategy, we confidently determined new lactylation, unveiling a wide landscape beyond histones from not only the enriched lactylproteome but also existing unenriched human proteome resources. Specifically, by mining the public human Meltome Atlas, we found that lactylation is common on glycolytic enzymes and conserved on ALDOA. We also discovered prevalent lactylation on DHRS7 in the draft of the human tissue proteome. We partially demonstrated the functional importance of lactylation: site-specific engineering of lactylation into ALDOA caused enzyme inhibition, suggesting a lactylation-dependent feedback loop in glycolysis.