Recently, a research team led by Zou Bingjie, Song Qinxin, and Wang Chen from the School of Pharmacy at our university published their findings titled “Voltage-Programmed Sequential Fluorescence Encoding (VPSFE) Enables Multiplexed In Situ Proteo-Imaging with Electric-Field Turing Patterns” in the internationally renowned journal Angewandte Chemie International Edition. Professor Zou Bingjie and Professor Song Qinxin from the School of Pharmacy at our university, along with Professor Zhou Guohua from Jinling Hospital affiliated with Nanjing University Medical School, served as co-corresponding authors. Wang Chen, a specially appointed associate researcher at the School of Pharmacy, is the first author and corresponding author, with China Pharmaceutical University listed as the first corresponding institution.
Single-cell spatiotemporal protein analysis technology is a crucial tool for drug target discovery and disease mechanism research. However, existing methods struggle to balance spatial resolution, sensitivity, and detection throughput. The research team developed a rapid exchange strategy for electrophoresis-based fluorescent probes. Combined with an immunolabeling oligonucleotide-based in situ signal amplification method, they established a new Turing pattern-encoded subcellular resolution multiplexed protein spatial imaging technique. This innovation enables in situ analysis of drug-related targets in cells and tissues. The team has consistently pursued novel analytical methods for detecting biomacromolecules like proteins, publishing related findings in analytical chemistry journals over the past three years (Anal. Chem. 2023, 95, 8621-8631; Anal. Chem. 2024, 96, 756-765; Biosens. Bioelectron. 2025, 289, 117864; Microchim. Acta. 2025, 192(9): 615).
This research was supported by the National Natural Science Foundation of China, the Jiangsu Provincial Key R&D Program for Social Development, and the Jiangsu Provincial Natural Science Foundation.
Paper link:
https://onlinelibrary.wiley.com/doi/10.1002/anie.202511629
Schematic Diagram of Turing Pattern Encoding and Single-Cell Multi-Target Specificity Imaging Results
